5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of <i>SULFATE TRANSPORTER 1;2</i> in <i>Arabidopsis</i> roots
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- Maruyama-Nakashita Akiko
- Graduate School of Agricultural Science, Kyushu University RIKEN Plant Science Center
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- Suyama Akiko
- Graduate School of Agricultural Science, Kyushu University
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- Takahashi Hideki
- RIKEN Plant Science Center Department of Biochemistry and Molecular Biology, Michigan State University
Bibliographic Information
- Other Title
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- 5'-non-transcribed flanking region and 5'-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in Arabidopsis roots
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Abstract
Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.
Journal
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- Plant Biotechnology
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Plant Biotechnology 34 (1), 51-55, 2017
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679305590016
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- NII Article ID
- 130005519596
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- HANDLE
- 2324/4377829
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- NDL BIB ID
- 028091806
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed