Generation of Small Colony Variants in Biofilms by Escherichia coli Harboring a Conjugative F Plasmid
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- Tashiro Yosuke
- Division of Environmental Engineering, Faculty of Engineering, Hokkaido University Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University
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- Eida Hiroaki
- Division of Environmental Engineering, Faculty of Engineering, Hokkaido University
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- Ishii Satoshi
- Division of Environmental Engineering, Faculty of Engineering, Hokkaido University Department of Soil, Water and Climate, University of Minnesota
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- Futamata Hiroyuki
- Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University
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- Okabe Satoshi
- Division of Environmental Engineering, Faculty of Engineering, Hokkaido University
書誌事項
- タイトル別名
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- Generation of Small Colony Variants in Biofilms by <i>Escherichia coli</i> Harboring a Conjugative F Plasmid
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<p>A conjugative F plasmid induces mature biofilm formation by Escherichia coli by promoting F-pili-mediated cell-cell interactions and increasing the expression of biofilm-related genes. We herein demonstrated another function for the F plasmid in E. coli biofilms; it contributes to the emergence of genetic and phenotypic variations by spontaneous mutations. Small colony variants (SCVs) were more frequently generated in a continuous flow-cell biofilm than in the planktonic state of E. coli harboring the F plasmid. E. coli SCVs represented typical phenotypic changes such as slower growth, less biofilm formation, and greater resistance to aminoglycoside antibiotics than the parent strain. Genomic and complementation analyses indicated that the small colony phenotype was caused by the insertion of Tn1000, which was originally localized in the F plasmid, into the hemB gene. Furthermore, the Tn1000 insertion was removed from hemB in the revertant, which showed a normal colony phenotype. This study revealed that the F plasmid has the potential to increase genetic variations not only by horizontal gene transfer via F pili, but also by site-specific recombination within a single cell.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 32 (1), 40-46, 2017
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679323846400
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- NII論文ID
- 130005519624
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 028095429
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- PubMed
- 28302951
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
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