Clostridium perfringens a-Toxin Impairs Lipid Raft Integrity in Neutrophils

• Takehara Masaya

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Takagishi Teruhisa

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Seike Soshi

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Oishi Kyohei

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Fujihara Yoshino

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Miyamoto Kazuaki

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Kobayashi Keiko

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Nagahama Masahiro

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

書誌事項

タイトル別名

• <i>Clostridium perfringens</i> α-Toxin Impairs Lipid Raft Integrity in Neutrophils
• Clostridium perfringens alpha-toxin impairs lipid raft integrity in neutrophils

抄録

Clostridium perfringens type A, a Gram-positive, anaerobic bacterium, causes gas gangrene. Recently, we reported that C. perfringens α-toxin blocked neutrophil differentiation in an enzyme activity-dependent manner to impair host innate immunity, which should be crucial for the pathogenesis of C. perfringens. However, the detailed mechanism remains unclear. Lipid rafts have been reported to be platforms for signaling molecules involved in the regulation of cell differentiation in many different cell types. In this study, we found that cell surface expression of a lipid raft marker, GM1 ganglioside, decreased in association with neutrophil differentiation by flow cytometry analysis and morphological observation. In vitro treatment of isolated mouse bone marrow cells with α-toxin or an α-toxin variant lacking phospholipase C and sphingomyelinase activities revealed that α-toxin increased the cell surface expression of GM1 ganglioside in an enzyme activity-dependent manner. C. perfringens infection also increased GM1 ganglioside levels in bone marrow myeloid cells. Moreover, treatment of bone marrow cells with methyl-β-cyclodextrin, a lipid raft-disrupting agent, impaired neutrophil differentiation. Together, our results suggest that the integrity of lipid rafts should be properly maintained during granulopoiesis, and α-toxin might perturb lipid raft integrity leading to the impairment of neutrophil differentiation.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 39 (10), 1694-1700, 2016

  公益社団法人 日本薬学会

キーワード

• <i>Clostridium perfringens</i> α-toxin
• lipid raft
• neutrophil differentiation

詳細情報

• CRID: 1390282679609463168
• NII論文ID: 130005598486
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b16-00444
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 027623925
• PubMed: 27725448
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027623925
• Web Site: https://www.jstage.jst.go.jp/article/bpb/39/10/39_b16-00444/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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