Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
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- TORAY Hisashi
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- HASEGAWA Tomoka
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- SAKAGAMI Naoko
- Divisions of Reconstructive Surgery for Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences
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- TSUCHIYA Erika
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- KUDO Ai
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- ZHAO Shen
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- MORITANI Yasuhito
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- ABE Miki
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- YOSHIDA Taiji
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- YAMAMOTO Tomomaya
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- YAMAMOTO Tsuneyuki
- Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University
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- ODA Kimimitsu
- Divisions of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences
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- UDAGAWA Nobuyuki
- Department of Biochemistry, Matsumoto Dental University
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- LUIZ de FREITAS Paulo Henrique
- Department of Dentistry, Federal University of Sergipe at Lagarto
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- LI Minqi
- Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University
書誌事項
- タイトル別名
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- <b>Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in </b><i><b>c-src</b></i><b> deficient </b><b>mice </b>
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抄録
<p>Since osteoblastic activities are believed to be coupled with osteoclasts, we have attempted to histologically verify which of the distinct cellular circumstances, the presence of osteoclasts themselves or bone resorption by osteoclasts, is essential for coupled osteoblastic activity, by examining c-fos−/− or c-src−/− mice. Osteopetrotic c-fos deficient (c-fos−/−) mice have no osteoclasts, while c-src deficient (c-src−/−) mice, another osteopetrotic model, develop dysfunctional osteoclasts due to a lack of ruffled borders. c-fos−/− mice possessed no tartrate-resistant acid phosphatase (TRAPase)-reactive osteoclasts, and showed very weak tissue nonspecific alkaline phosphatase (TNALPase)-reactive mature osteoblasts. In contrast, c-src−/− mice had many TNALPase-positive osteoblasts and TRAPase-reactive osteoclasts. Interestingly, the parallel layers of TRAPase-reactive/osteopontin-positive cement lines were observed in the superficial region of c-src−/− bone matrix. This indicates the possibility that in c-src−/− mice, osteoblasts were activated to deposit new bone matrices on the surfaces that osteoclasts previously passed along, even without bone resorption. Transmission electron microscopy demonstrated cell-to-cell contacts between mature osteoblasts and neighboring ruffled border-less osteoclasts, and osteoid including many mineralized nodules in c-src−/− mice. Thus, it seems likely that osteoblastic activities would be maintained in the presence of osteoclasts, even if they are dysfunctional.</p>
収録刊行物
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- Biomedical Research
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Biomedical Research 38 (2), 123-134, 2017
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詳細情報 詳細情報について
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- CRID
- 1390282679879466240
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- NII論文ID
- 130005606444
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- NII書誌ID
- AA00110128
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- ISSN
- 1880313X
- 03886107
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- HANDLE
- 2115/80591
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- PubMed
- 28442663
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
