<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>
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- KAWANO Eisuke
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- TORIUMI Taku
- Department of Anatomy, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
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- IGUCHI Shinya
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- SUZUKI Daigo
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- SATO Shuichi
- Department of Periodontology, Nihon University School of Dentistry Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry
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- HONDA Masaki
- Department of Oral Anatomy, Aichi-Gakuin University School of Dentistry
Bibliographic Information
- Other Title
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- Induction of neural crest cells from human dental pulp-derived induced pluripotent stem cells
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Abstract
<p>We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres. The neurospheres were transferred to fibronectin-coated dishes and formed rosette structures. The migrated cells from the rosettes abundantly expressed NCC markers, as evidenced by real-time polymerase chain reaction, immunofluorescence, and flow cytometric analysis. Furthermore, the migrated cells exhibited the ability to differentiate into neural crest lineage cells in vitro. They also exhibited tissue-forming potential in vivo, differentiating into bone and cartilage. Collectively, the migrated cells had similar characteristics to those of NCCs. These results suggest that human dental pulp cell-derived iPS cells are capable of differentiating into NCCs. Therefore, iPS cell-derived NCCs represent cell sources for bone and cartilage tissue engineering.</p>
Journal
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- Biomedical Research
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Biomedical Research 38 (2), 135-147, 2017
Biomedical Research Press