The elongation factor Tu from  <i>Lactobacillus reuteri</i>  inhibits the adhesion of  <i>Helicobacter pylori</i> to porcine gastric mucin

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  • Nishiyama Keita
    Department of Microbiology, School of Pharmacy, Kitasato University
  • Kagamitani Takeo
    Department of Animal Science, Kitasato University School of Veterinary Medicine
  • Yamamoto Yuji
    Department of Animal Science, Kitasato University School of Veterinary Medicine
  • Okada Nobuhiko
    Department of Microbiology, School of Pharmacy, Kitasato University
  • Mukai Takao
    Department of Animal Science, Kitasato University School of Veterinary Medicine

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Other Title
  • <i>Lactobacillus reuteri</i>由来Elongation factor Tuはブタ胃ムチンに対する<i>Helicobacter pylori</i>の付着を阻害する
  • The elongation factor Tu from Lactobacillus reuteri inhibits the adhesion of Helicobacter pylori to porcine gastric mucin

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Abstract

<p> Helicobacter pylori is a Gram-negative spiral-shaped microaerophilic bacterium that infects more than half of the world's population. Probiotic treatment may inhibit H. pylori infection by competition with the pathogen for the same receptor site (s) on the host mucosal surface. We previously found that Lactobacillus reuteri JCM1081 cell surface-associated elongation factor Tu (EF-Tu) was characterized as a sulfated carbohydrate-binding protein and may mediate adhesion of H. pylori to the host gastrointestinal tract. Here, we evaluated the role of EF-Tu in inhibiting H. pylori adhesion, using recombinant EF-Tu protein (His-EF-Tu). The addition of His-EF-Tu showed concentration-dependent inhibitory effects on the adhesion of H. pylori strains to porcine gastric mucin (PGM)-coated wells. Similar inhibitory effects were observed in H. pylori clinical isolates. Interestingly, sulfatase-treated PGM reduced H. pylori adhesion, whereas His-EF-Tu barely affected H. pylori adhesion on sulfatase-treated PGM. The inhibitory rate was similar to that of pretreatment with the sulfated carbohydrate recognition antibody PGM34. Western blotting revealed that EF-Tu was present in cell surface fractions isolated from several Lactobacillus strains. Thus, EF-Tu could inhibit the adhesion of several H. pylori strains, and the inhibitory effect may be mediated through competition of EF-Tu and H. pylori ligands for the same adhesion sites.</p>

Journal

  • Milk Science

    Milk Science 66 (1), 17-26, 2017

    Japanese Dairy Science Association

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