<i>In Situ</i> Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions

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Author(s)

    • Iwabuchi Erina
    • Department of Pathology, Tohoku University Graduate School of Medicine
    • Miki Yasuhiro
    • Department of Disaster Obstetrics and Gynecology, International Research Institute of Disaster Science (IRIDes), Tohoku University
    • Ono Katsuhiko
    • Department of Pathology, Tohoku University Graduate School of Medicine
    • Sasano Hironobu
    • Department of Pathology, Tohoku University Graduate School of Medicine

Abstract

<p>The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by <i>in situ</i> methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both homodimers and heterodimers in breast carcinoma cell lines using Nikon's SIM (N-SIM). ERα/α homodimers were detected in the nuclei of both ERα-positive MCF-7 and T-47D cells; 23.0% and 13.4% of ERα proteins formed ERα/α homodimers, respectively. ERα/β heterodimers were also detected in MCF-7 and T-47D. Approximately 6.6% of both ERα and ERβ1 proteins formed ERα/β1 heterodimers in MCF-7. In addition, 18.1% and 22.4% of ERα and ERβ proteins formed ERα/β2 heterodimers and ERα/β5 heterodimers in MCF-7, respectively. In addition, by using proximity ligation assay (PLA) in MCF-7, estradiol-induced ERα/α homodimers and ERα/β1 heterodimers were both detected after 15 to 45 min of treatment and at 15 min, respectively. The percentage of total ER proteins could also be determined using N-SIM. By using both methods, it has become possible to evaluate precise localization and ratio of ER dimers among different cell types.</p>

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 50(2), 85-93, 2017

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Codes

  • NII Article ID (NAID)
    130005621182
  • Text Lang
    ENG
  • ISSN
    0044-5991
  • Data Source
    J-STAGE 
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