Intranasal administration of IL-35 inhibits allergic responses and symptoms in mice with allergic rhinitis

• Suzuki Motohiko

• Yokota Makoto

  Department of Otorhinolaryngology, Nagoya City University

• Nakamura Yoshihisa

  Department of Otorhinolaryngology, Nagoya City University

• Ozaki Shinya

  Department of Otorhinolaryngology, Nagoya City University

• Murakami Shingo

  Department of Otorhinolaryngology, Nagoya City University

Abstract

Background: IL-35 was recently identified as an anti-inflammatory cytokine. We previously reported that recombinant fusion protein of murine IL-35 and human IgG1 Fc fragment (rIL-35) reduced Th2 cytokines (IL-4 and IL-5) in vitro. However, it is unclear whether IL-35 can attenuate nasal allergic responses and symptoms of allergic rhinitis in vivo. Methods: To investigate the in vivo effect of IL-35 on allergic rhinitis in mice, mice were sensitized with ovalbumin (OVA). Intranasal administration of rIL-35 and intranasal challenge of OVA were then performed. Nasal symptoms were estimated after the last nasal challenge. Nasal tissue and cervical lymph nodes (CLN) were collected. OVA-specific IgE in sera, OVA-specific T cell response, and the production of cytokines (IL-4, IL-5, and IL-10) stimulated by the OVA antigen were measured. The transcription level of Foxp3 and the frequency of CD4⁺CD25⁺ regulatory T cells were also measured. Results: rIL-35 significantly inhibited the number of sneezes and nasal rubbing movements. It also reduced the number of eosinophils in the nasal mucosa and significantly decreased the level of OVA-specific IgE, the OVA-specific T cell proliferation, and the production of IL-4 and IL-5. Furthermore, rIL-35 significantly increased the production of IL-10, the transcription level of Foxp3, and the frequency of CD4⁺CD25⁺ regulatory T cells. Conclusions: This study showed for the first time that rIL-35 inhibits nasal allergic responses and symptoms in mice, and that rIL-35 increases IL-10, Foxp3, and CD4⁺CD25⁺ regulatory T cells in CLN. This study also suggests that intranasal administration of IL-35 can attenuate allergic rhinitis.

Journal

• Allergology International

  Allergology International 66 (2), 351-356, 2017

  Japanese Society of Allergology

Keywords

• Allergic rhinitis
• Cervical lymph node
• IL-10
• IL-35
• Regulatory T cell

Details

• CRID: 1390282679608731520
• NII Article ID: 130005628187
• DOI: 10.1016/j.alit.2016.08.014
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016301332?httpAccept=text/plain
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016301332?httpAccept=text/xml
• Web Site: https://search.jamas.or.jp/link/ui/2018000523
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed