Circulating activated innate lymphoid cells and mucosal-associated invariant T cells are associated with airflow limitation in patients with asthma

• Ishimori Ayako

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Harada Norihiro

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine Research Institute for Diseases of Old Ages, Juntendo University Faculty of Medicine and Graduate School of Medicine Atopy (Allergy) Research Center, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Chiba Asako

  Department of Immunology, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Harada Sonoko

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine Research Institute for Diseases of Old Ages, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Matsuno Kei

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Makino Fumihiko

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Ito Jun

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Ohta Shoichiro

  Department of Laboratory Medicine, Saga Medical School

• Ono Junya

  Shino-Test Corporation

• Atsuta Ryo

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Izuhara Kenji

  Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School

• Takahashi Kazuhisa

  Department of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine

• Miyake Sachiko

  Department of Immunology, Juntendo University Faculty of Medicine and Graduate School of Medicine

Abstract

Background: A variety of innate subsets of lymphoid cells such as natural killer (NK) cells, several populations of innate lymphoid cells (ILCs), and mucosal-associated invariant T (MAIT) cells as innate-like T lymphocytes are involved in asthma and may have important effector functions in asthmatic immune responses. In the present study, we investigated whether NK cells, ILCs, and MAIT cells in the peripheral blood of patients with asthma would be associated with clinical asthma parameters. Methods: We recruited 75 adult patients with mild to severe asthma. The peripheral blood mononuclear cells in peripheral venous blood samples from the patients were purified and stained with different combinations of appropriate antibodies. The cells were analyzed by flow cytometry. Results: The percentage of activated (i.e., CD69⁺) NK cells in the total NK cell population was negatively correlated with FEV₁% which is calculated by the forced expiratory volume in 1 s (FEV₁)/the forced vital capacity (FVC). The percentages of CD69⁺ ILC1s and ILC2s were negatively correlated with FEV₁% and %FEV₁. The percentage of CD69⁺ ILC3s was positively correlated with BMI, and the percentage of CD69⁺ MAIT cells was negatively correlated with FEV₁%. Moreover, the percentage of CD69⁺ NK cells, ILC1s, ILC2s, ILC3s, and MAIT cells were positively correlated with each other. Conclusions: For the first time, our data showed that activated NK cells, ILC1s, ILC2s, ILC3s, and MAIT cells were positively correlated with each other and may be associated with airflow limitation in patients with asthma.

Journal

• Allergology International

  Allergology International 66 (2), 302-309, 2017

  Japanese Society of Allergology

Keywords

• Airflow limitation
• Asthma
• Innate lymphoid cell
• Mucosal-associated invariant T cell
• Natural killer cell

Details

• CRID: 1390001204631199488
• NII Article ID: 130005628231
• DOI: 10.1016/j.alit.2016.07.005
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016301058?httpAccept=text/plain
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016301058?httpAccept=text/xml
• Web Site: https://search.jamas.or.jp/link/ui/2018000516
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed