清拭タオルの<i>Bacillus cereus</i>汚染を高感度に検出する改良ビーズ抽出法  [in Japanese] Highly Sensitive Modified Beads Extraction Method for the Detection of the Contamination of Reused Hand Towels with Bacillus Cereus Spores  [in Japanese]

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Abstract

<p><i>Bacillus cereus</i>による敗血症の多くは,清拭タオルの<i>B. cereus</i>芽胞汚染から患者皮膚・医療者の手指などを介した輸液汚染が原因である.したがって防止対策には清拭タオルの<i>B. cereus</i>汚染状況の把握が必要だが,汚染の定量的測定法が確立されているとは言えない.そこで検出率を高めるためにビーズ抽出法を改良し,清拭タオルの<i>B. cereus</i>汚染を調査した.業者から納入された洗浄・乾燥済み清拭タオルを無作為に選択し,改良法により1枚のタオルから23か所の切片を切り出し各切片についてビーズ抽出法を行った結果,9枚中7枚に<i>B. cereus</i>の検出がみられた.汚染は辺縁部位に比較的多くみられた.</p>

<p>Contamination of reused hand towels by <i>Bacillus cereus</i> spores sometimes causes severe sepsis, and treatment of the towels with disinfectants in the washing process hardly removes the contamination. Evaluation of the degree of the spore contamination of the towels may require the quantification of the contamination although a standard method for it has not been established yet. Therefore, we developed a modified bead extraction method to improve the detection rate of the contamination and then applied the method to measure the contamination with <i>B. cereus</i> of hand towels obtained from a university hospital. Test objects were randomly selected from the washed and dried towels delivered from a laundry company. We cut off 23 pieces from each towel and extracted bacteria from the pieces with the modified method. We found <i>B. cereus</i> contamination of one or more pieces obtained from 7 out of 9 towels. The contamination was rather frequently detected in the pieces from the fringe of the towels that were expected to be clean. Therefore, it is likely that the modified bead extraction method effectively detected <i>B. cereus</i> spore contamination. We also performed scanning electron microscopy to detect spores on the surface of towels that were soaked with <i>B. cereus</i> spore solution. We found <i>B. cereus</i> spores preferentially adhering to niches and wrinkles of towel fibers. Accordingly, we recommend the use of disposable hand towels.</p>

Journal

  • Japanese Journal of Infection Prevention and Control

    Japanese Journal of Infection Prevention and Control 32(2), 85-88, 2017

    Japanese Society for Infection Prevention and Control

Codes

  • NII Article ID (NAID)
    130005655704
  • NII NACSIS-CAT ID (NCID)
    AA12313188
  • Text Lang
    JPN
  • ISSN
    1882-532X
  • NDL Article ID
    028091159
  • NDL Call No.
    Z19-3650
  • Data Source
    NDL  J-STAGE 
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