Association between Intraosseous Vasculature and Bone Matrix Anisotropy Analyzed with a Novel Visualization Method

• Sekita Aiko

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• Matsugaki Aira

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• Ozasa Ryosuke

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• Nakano Takayoshi

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

Bibliographic Information

Other Title

• 新規イメージング法による骨内血管網－骨基質異方性構造の相関解明
• シンキ イメージングホウ ニ ヨル ホネ ナイ ケッカンモウ-コツ キシツ イホウセイ コウゾウ ノ ソウカン カイメイ

Abstract

Bone tissue has a highly anisotropic microstructure derived from collagen matrix alignment and the crystallographic orientation of apatite. Bone is also a highly vascularized tissue; intraosseous vascularization and bone formation are intimately coupled. Meanwhile, the structural relations between intraosseous vascular networks and bone microstructure are as yet unknown, partially due to technical difficulties in visualizing precise intraosseous vasculatures. The aim of this study is to develop a visualization method suitable for the structural analysis of intraosseous vascular networks and to reveal the relations between bone microstructure and the arrangement patterns of intraosseous vasculatures both in intact and metastasized bones. Three-dimensional vascular networks were successfully visualized, and region-dependent arrangement patterns of blood vessels were clarified using fluorescent dye-conjugated lectin. Interestingly, the anisotropic structural correlation between bone matrix and the vascular system in a region-specific manner was clarified. In addition, the impaired anisotropic vascular networks in metastasized bone was clarified. The obtained results indicate the molecular interactions between the vascular system and bone tissue as a novel contributor for realization of anisotropic bone matrix construct.

Journal

• Journal of the Japan Institute of Metals and Materials

  Journal of the Japan Institute of Metals and Materials 81 (6), 315-319, 2017

  The Japan Institute of Metals and Materials

Keywords

• bone tissue anisotropy
• correlation between bone matrix and vascular system
• intraosseous vascular networks
• three-dimensional visualization
• structural patterns
• longitudinal alignment
• bone metastasis

Details

• CRID: 1390001206491153536
• NII Article ID: 130005678931
• NII Book ID: AN00187860
• DOI: 10.2320/jinstmet.j2017005
• ISSN: 18806880
• ISSN: 24337501
• ISSN: 00214876
• NDL BIB ID: 028305762
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028305762
• Web Site: https://www.jstage.jst.go.jp/article/jinstmet/81/6/81_J2017005/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed