Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae

• Ozasa Ryosuke

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

• Matsugaki Aira

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

• Isobe Yoshihiro

  Atree, Inc

• Saku Taro

  Atree, Inc

• Nakano Takayoshi

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

抄録

Bone tissue has a highly anisotropic microstructure comprised of biological apatite (BAp) and collagen fibrils, which is closely related to bone mechanical function. The formation of anisotropic bone microstructure is governed by bone-forming osteoblasts; therefore, isolation of functional osteoblasts and regulation of their arrangement for generating bone tissue with optimally-oriented microstructure during bone reconstruction are important. In this study, we established the isolation and culture conditions of mature osteoblasts derived from juvenile mice (2-week-old). Osteoblasts from juvenile mice expressed significantly higher level of osteoblastic markers (alkaline phosphatase, osterix, and osteocalcin) than osteoblasts from neonatal mice, indicating that juvenile osteoblasts are promising materials for bone tissue engineering. Moreover, alignment of the mature osteoblasts was successfully regulated using oriented collagen substrates. This is the report that shows directional control of mature osteoblasts isolated from juvenile mice.

収録刊行物

• MATERIALS TRANSACTIONS

  MATERIALS TRANSACTIONS 58 (6), 958-962, 2017

  公益社団法人 日本金属学会

キーワード

• bone anisotropy
• primary osteoblast
• isolation method
• mature cell
• cell orientation
• oriented collagen substrate

詳細情報

• CRID: 1390282679231651968
• NII論文ID: 130005679019
• NII書誌ID: AA1151294X
• DOI: 10.2320/matertrans.m2017077
• ISSN: 13475320
• ISSN: 13459678
• NDL書誌ID: 028195768
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028195768
• Web Site: https://www.jstage.jst.go.jp/article/matertrans/58/6/58_M2017077/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可