Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae

Access this Article

Search this Article

Author(s)

Abstract

<p>Bone tissue has a highly anisotropic microstructure comprised of biological apatite (BAp) and collagen fibrils, which is closely related to bone mechanical function. The formation of anisotropic bone microstructure is governed by bone-forming osteoblasts; therefore, isolation of functional osteoblasts and regulation of their arrangement for generating bone tissue with optimally-oriented microstructure during bone reconstruction are important. In this study, we established the isolation and culture conditions of mature osteoblasts derived from juvenile mice (2-week-old). Osteoblasts from juvenile mice expressed significantly higher level of osteoblastic markers (alkaline phosphatase, osterix, and osteocalcin) than osteoblasts from neonatal mice, indicating that juvenile osteoblasts are promising materials for bone tissue engineering. Moreover, alignment of the mature osteoblasts was successfully regulated using oriented collagen substrates. This is the report that shows directional control of mature osteoblasts isolated from juvenile mice.</p>

Journal

  • MATERIALS TRANSACTIONS

    MATERIALS TRANSACTIONS 58(6), 958-962, 2017

    The Japan Institute of Metals and Materials

Codes

  • NII Article ID (NAID)
    130005679019
  • NII NACSIS-CAT ID (NCID)
    AA1151294X
  • Text Lang
    ENG
  • ISSN
    1345-9678
  • NDL Article ID
    028195768
  • NDL Call No.
    Z53-J286
  • Data Source
    NDL  J-STAGE 
Page Top