Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae

• Ozasa Ryosuke

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

• Matsugaki Aira

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

• Isobe Yoshihiro

  Atree, Inc

• Saku Taro

  Atree, Inc

• Nakano Takayoshi

  Division of Materials Science and Engineering, Graduate School of Engineering, Osaka University

Abstract

Bone tissue has a highly anisotropic microstructure comprised of biological apatite (BAp) and collagen fibrils, which is closely related to bone mechanical function. The formation of anisotropic bone microstructure is governed by bone-forming osteoblasts; therefore, isolation of functional osteoblasts and regulation of their arrangement for generating bone tissue with optimally-oriented microstructure during bone reconstruction are important. In this study, we established the isolation and culture conditions of mature osteoblasts derived from juvenile mice (2-week-old). Osteoblasts from juvenile mice expressed significantly higher level of osteoblastic markers (alkaline phosphatase, osterix, and osteocalcin) than osteoblasts from neonatal mice, indicating that juvenile osteoblasts are promising materials for bone tissue engineering. Moreover, alignment of the mature osteoblasts was successfully regulated using oriented collagen substrates. This is the report that shows directional control of mature osteoblasts isolated from juvenile mice.

Journal

• MATERIALS TRANSACTIONS

  MATERIALS TRANSACTIONS 58 (6), 958-962, 2017

  The Japan Institute of Metals and Materials

Keywords

• bone anisotropy
• primary osteoblast
• isolation method
• mature cell
• cell orientation
• oriented collagen substrate

Details

• CRID: 1390282679231651968
• NII Article ID: 130005679019
• NII Book ID: AA1151294X
• DOI: 10.2320/matertrans.m2017077
• ISSN: 13475320
• ISSN: 13459678
• NDL BIB ID: 028195768
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028195768
• Web Site: https://www.jstage.jst.go.jp/article/matertrans/58/6/58_M2017077/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed