Development of a microfluidic in vitro model of tumor cell invasion

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  • TACHIKAWA Saeko
    東京大学大学院　工学系研究科
  • KANEDA Shohei
    東京大学生産技術研究所　LIMMS/CNRS-IIS 東京大学生産技術研究所　統合バイオメディカルシステム国際研究センター
  • KUMEMURA Momoko
    東京大学生産技術研究所　LIMMS/CNRS-IIS 東京大学生産技術研究所　マイクロナノ学際研究センター
  • SATO Rui
    群馬大学
  • FUJII Teruo
    東京大学生産技術研究所　統合バイオメディカルシステム国際研究センター
  • SUZUKI Takaaki
    群馬大学
  • FUJITA Hiroyuki
    東京大学生産技術研究所　マイクロナノ学際研究センター

Bibliographic Information

Other Title
  • マイクロ流体<i>in vitro</i>がん浸潤モデルの構築
  • マイクロ流体in vitroがん浸潤モデルの構築
  • マイクロ リュウタイ in vitro ガン シンジュン モデル ノ コウチク

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Abstract

<p>In this research, we developed a microfluidic in vitro cancer invasion model which takes into account both intercellular adhesion and cell-cell interaction existing in 3D tumor cell clusters. The device has 2 parallel main channels connected with 10 orifices which have taper-shaped structures. The diameters of wider openings of the orifice and its narrower pore are approximately 80 μm, and 10 μm respectively. The wide opening allows several cells to be packed in the orifice and to touch each other tightly. The device made of PDMS was fabricated through two-step softlithography using an SU-8 mold produced by a 3D lithography technique. In device evaluation, PC-3 cells （a prostate cancer cell line） introduced from one main channel were packed into the orifices using a flow towards the other main channel generated by hydrostatic pressure difference. Then by filling the other main channel with a medium containing invasion-inducing factors, an invasion cell moved through the pore along the laminin-coated wall of the orifice was successfully observed on the device.</p>

Journal

  • SEISAN KENKYU

    SEISAN KENKYU 69 (3), 137-140, 2017

    Institute of Industrial Science The University of Tokyo

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