Effect of DNA Methylation on the Velocity of DNA Translocation through a Nanochannel

• SUN Xiaoyin

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University ImPACT Research Center for Advanced Nanobiodevices, Nagoya University

• YASUI Takao

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University ImPACT Research Center for Advanced Nanobiodevices, Nagoya University Japan Science and Technology Agency (JST), PRESTO

• YANAGIDA Takeshi

  Institute of Materials Chemistry and Engineering, Kyushu University Institute of Scientific and Industrial Research, Osaka University

• KAJI Noritada

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University ImPACT Research Center for Advanced Nanobiodevices, Nagoya University Japan Science and Technology Agency (JST), PRESTO

• RAHONG Sakon

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University ImPACT Research Center for Advanced Nanobiodevices, Nagoya University

• KANAI Masaki

  Institute of Materials Chemistry and Engineering, Kyushu University

• NAGASHIMA Kazuki

  Institute of Materials Chemistry and Engineering, Kyushu University

• KAWAI Tomoji

  Institute of Scientific and Industrial Research, Osaka University

• BABA Yoshinobu

  Department of Applied Chemistry, Graduate School of Engineering, Nagoya University ImPACT Research Center for Advanced Nanobiodevices, Nagoya University Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

抄録

Here, we report the effect of DNA methylation on the velocity of DNA translocation through a nanochannel, as determined by measuring differences in translocation velocities between methylated and non-methylated DNA molecules. We found that the velocity of translocation of methylated DNA was faster than that of non-methylated DNA, which we attributed to variation in the coefficients of diffusion and friction with the nanochannel wall, due to the increased molecular weight and stiffness, respectively, of methylated DNA.

収録刊行物

• Analytical Sciences

  Analytical Sciences 33 (6), 727-730, 2017

  社団法人 日本分析化学会

キーワード

• DNA methylation
• single DNA molecule
• DNA translocation velocity
• nanochannel

詳細情報

• CRID: 1390001204260766080
• NII論文ID: 130005696867
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.33.727
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028299238
• PubMed: 28603194
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028299238
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.727.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.727/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/6/33_727/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018145502
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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