Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

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Author(s)

    • Fujii Sumie
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital|Department of Hematology/Oncology, Graduate School of Medicine, Kyoto University
    • Ichinohe Tatsuo
    • Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University
    • Maekawa Taira
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital
    • Miura Yasuo
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital
    • Iwasa Masaki
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital|Division of Gastroenterology and Hematology, Shiga University of Medical Science
    • Yoshioka Satoshi
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital
    • Fujishiro Aya
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital|Division of Gastroenterology and Hematology, Shiga University of Medical Science
    • Sugino Noriko
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital|Department of Hematology/Oncology, Graduate School of Medicine, Kyoto University
    • Nakagawa Yoko
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital
    • Hirai Hideyo
    • Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital

Abstract

<p>Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10<sup>8</sup>) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells. The number of nucleated cells before and after hydroxyethyl starch separation, UCB unit volume, and cell viability after thawing did not significantly differ between UCB units from which MSCs were successfully isolated and those from which they were not. When CryoSure-DEX40 was used as a cryoprotectant, MSCs were isolated from two of ten UCB units. Logistic regression analysis demonstrated that the cryopreservation method was not significantly associated with the success of MSC isolation. The isolated MSCs had a similar morphology and surface marker expression profile as bone marrow-derived MSCs and were able to differentiate into osteogenic, adipogenic, and chondrogenic cells. In summary, MSCs can be isolated from cryopreserved UCB cells. However, the cryopreservation process reduces the isolation rate; therefore, freshly donated UCB cells are preferable for the isolation of MSCs.</p>

Journal

  • Journal of Clinical and Experimental Hematopathology

    Journal of Clinical and Experimental Hematopathology 57(1), 1-8, 2017

    The Japanese Society for Lymphoreticular Tissue Research

Codes

  • NII Article ID (NAID)
    130005709558
  • Text Lang
    ENG
  • ISSN
    1346-4280
  • Data Source
    J-STAGE 
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