Analysis of deposited peptides in amyloid lesions of AL amyloidosis by mass spectrometry

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  • SUGIMOTO Toshiya
    Graduate School of Health Sciences, Kumamoto University
  • TASAKI Masayoshi
    Division of Advanced Biomedical Sciences, Kumamoto University Department of Neurology, Faculty of Medical Sciences, Kumamoto University
  • YAMASHITA Taro
    Amyloidosis Diagnosis Unit, Kumamoto University Hospital
  • UEDA Mitsuharu
    Department of Neurology, Faculty of Medical Sciences, Kumamoto University
  • ANDO Yukio
    Department of Neurology, Faculty of Medical Sciences, Kumamoto University
  • OBAYASHI Konen
    Division of Advanced Biomedical Sciences, Kumamoto University
  • OKUNO Yutaka
    Department of Hematology, Faculty of Medical Sciences, Kumamoto University
  • HATA Hiroyuki
    Department of Hematology, Faculty of Medical Sciences, Kumamoto University Department of Informative Clinical Sciences, Faculty of Medical Sciences, Kumamoto University

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Other Title
  • ALアミロイドーシスにおける質量分析を用いた沈着ペプチド解析
  • 臨床研究 ALアミロイドーシスにおける質量分析を用いた沈着ペプチド解析
  • リンショウ ケンキュウ AL アミロイドーシス ニ オケル シツリョウ ブンセキ オ モチイタ チンチャク ペプチド カイセキ

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Abstract

<p>Although immunoglobulin light chain (LC) plays a critical role in AL amyloidosis, detailed characteristics of deposited LC are not well known. In this study, LC peptides were analyzed by a combination of laser microdissection, liquid chromatography, and mass spectrometry (LC-MS/MS) in 65 patients with AL amyloidosis. Constant regions of LC were detected in all patients. Kappa- or lambda-types were detected in 20 and 45 patients, respectively. Various types of constant regions of LC were found; however, IGLC3 and IGKC4 were the most frequently detected. Besides LC, apolipoproteins and vitronectin were repeatedly found in amyloid lesions. These results suggest marked heterogeneity in terms of subtype of constant regions of LC in amyloid lesions. Immunohistochemistry identified LC in approximately half of patients in whom LC was detected by LC-MS/MS. This finding indicates superiority of LC-MS/MS over IHC for the detection of LC.</p>

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 58 (6), 589-594, 2017

    The Japanese Society of Hematology

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