Basic research for development of ultrasonic prevention of vascular occlusion

• SAWAGUCHI Yoshikazu

  Department of Clinical Pharmaceutics, Nihon Pharmaceutical University Division of Ultrasound Device Development and Application (DOUDA), the Jikei University School of Medicine

• WANG Zuojun

  Division of Ultrasound Device Development and Application (DOUDA), the Jikei University School of Medicine

• ITOU Souma

  Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

• KIKUCHI Ran

  Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

• YAMAMOTO Hiroyuki

  Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University

• TACHIBANA Ken

  Department of Health Biosciences, Nihon Pharmaceutical University

• NAKAJIMA Takanori

  Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

• NAKATA Norio

  Division of Ultrasound Device Development and Application (DOUDA), the Jikei University School of Medicine

Bibliographic Information

Other Title

• 超音波による血管閉塞予防法開発のための基礎的検討

Abstract

Background: Reocclusion occurred after various recanalization therapies. In particular, reocclusion was frequently observed immediately after the recombinant tissue-type plasminogen activator (rt-PA) treatment. This is a fatal problem, because anticoagulant therapy is prohibited within 24 hours after the rt-PA treatment. We report the thrombus growth control effect of non-invasive ultrasound (US) in an in vitro clot growth model.<br> Method: A clot was prepared by incubation of citrated human plasma, CaCl₂ solution and thrombin at 37°C for 60 min in a latex tube. After incubation, the latex tube was filled with degassed citrated human plasma. The plasma around the clot was irradiated with US in a water bath at 37°C. The US irradiation condition was: transducer size 10 mm, frequency 500 kHz, continuous wave, average intensity 0.32 W/cm², 30 min. To evaluate the thrombus growth control effect, the clot’s weight was measured before and after US irradiation. <br> Results: The clot’s increased weight was significantly suppressed in the US group compared with the Non-US group (58.3 mg vs. 107.7 mg, p<0.0025). <br> Conclusions: In this study, we showed that the non-invasive US could control the growth of thrombus. This safe and simple US method may be used to prevent the reocclusion after recanalization therapy.

Journal

• Neurosonology

  Neurosonology 30 (1), 4-7, 2017

  The Japan Academy of Neurosonology

Keywords

• non-invasive ultrasound
• rt-PA
• reocclusion
• prevention

Details

• CRID: 1390001204421642624
• NII Article ID: 130005757719
• DOI: 10.2301/neurosonology.30.4
• ISSN: 18843336
• ISSN: 0917074X
• Web Site: https://www.jstage.jst.go.jp/article/neurosonology/30/1/30_4/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018092767
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed