Determination of Menadione by Liquid Chromatography–Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring

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Author(s)

Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 – 8.2%, with the corresponding recoveries equaling 90.5 – 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

Journal

  • Analytical Sciences

    Analytical Sciences 33(7), 863-867, 2017

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130005796381
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    028340179
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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