Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial
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- YANG Longqiang
- Department of Pediatric Dentistry, Tokyo Dental College Oral Health Science Center, Tokyo Dental College
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- TANABE Koji
- Oral Health Science Center, Tokyo Dental College Department of Pharmacology, Tokyo Dental College
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- MIURA Tadashi
- Oral Health Science Center, Tokyo Dental College
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- YOSHINARI Masao
- Oral Health Science Center, Tokyo Dental College
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- TAKEMOTO Shinji
- Oral Health Science Center, Tokyo Dental College Department of Dental Materials Science, Tokyo Dental College
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- SHINTANI Seikou
- Department of Pediatric Dentistry, Tokyo Dental College
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- KASAHARA Masataka
- Department of Pharmacology, Tokyo Dental College
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抄録
<p>This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (−30, −80 and −196oC), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro. Freezing temperature did not affect porosity while high freezing temperature (−30oC) increased pore size. The high gelatin concentration group (0.6%AC+2.4%G) had decreased porosity and pore size. Freezing time and 500 μM fluvastatin did not affect pore structures. The cytotoxicity and cell proliferation assays revealed that ACG sponges had no cytotoxic effects on human mesenchymal stromal cell growth and proliferation. These results indicate that ACG sponge may be a good biomaterial scaffold for bone regeneration.</p>
収録刊行物
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- Dental Materials Journal
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Dental Materials Journal 36 (4), 429-437, 2017
日本歯科理工学会