The Involvement of the Oxidative Stress in Murine Blue LED Light-Induced Retinal Damage Model

• Nakamura Maho

  Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University

• Kuse Yoshiki

  Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University

• Tsuruma Kazuhiro

  Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University

• Shimazawa Masamitsu

  Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University

• Hara Hideaki

  Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University

抄録

The aim of study was to establish a mouse model of blue light emitting diode (LED) light-induced retinal damage and to evaluate the effects of the antioxidant N-acetylcysteine (NAC). Mice were exposed to 400 or 800 lx blue LED light for 2 h, and were evaluated for retinal damage 5 d later by electroretinogram amplitude and outer nuclear layer (ONL) thickness. Additionally, we investigated the effect of blue LED light exposure on shorts-wave-sensitive opsin (S-opsin), and rhodopsin expression by immunohistochemistry. Blue LED light induced light intensity dependent retinal damage and led to collapse of S-opsin and altered rhodopsin localization from inner and outer segments to ONL. Conversely, NAC administered at 100 or 250 mg/kg intraperitoneally twice a day, before dark adaptation and before light exposure. NAC protected the blue LED light-induced retinal damage in a dose-dependent manner. Further, blue LED light-induced decreasing of S-opsin levels and altered rhodopsin localization, which were suppressed by NAC. We established a mouse model of blue LED light-induced retinal damage and these findings indicated that oxidative stress was partially involved in blue LED light-induced retinal damage.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 40 (8), 1219-1225, 2017

  公益社団法人 日本薬学会

キーワード

• blue light emitting diode
• age-related macular degeneration
• <i>N</i>-acetylcysteine

詳細情報

• CRID: 1390001204631251712
• NII論文ID: 130005876337
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b16-01008
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 028388345
• PubMed: 28769003
• Web Site: http://id.ndl.go.jp/bib/028388345
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028388345
• Web Site: https://www.jstage.jst.go.jp/article/bpb/40/8/40_b16-01008/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018114604
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可