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- Kubicek-Sutherland Jessica Z.
- Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory
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- Hengartner Astrid C.
- Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory
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- Mukundan Harshini
- Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory
抄録
<p>The incorrect diagnosis of bacterial pathogens results in the misuse of antibiotics, a major contributor to the evolution of antibiotic resistance. The ability to rapidly identify a pathogen could facilitate more effective treatment strategies, reducing the unnecessary use of antibiotics. Current diagnostics of bacterial infections often require cell growth, and are therefore less capable of informing timely treatment options. Here, we describe the development of a rapid assay (<45 min) to identify Gram-positive bacteria directly in human serum without any growth or isolation required. We have developed a fluorescence-based immunoassay specifically targeting the Gram-positive bacterial biomarker lipoteichoic acid (LTA). LTA is an amphiphilic molecule with both hydrophobic and hydrophilic portions. Our approach, termed membrane insertion, exploits the amphiphilic biochemistry of the molecule by allowing it to passively insert itself into a lipid bilayer, thereby trapping it for subsequent probing with a fluorescently labeled α-LTA antibody. Using this assay, we have successfully detected less than 10 μg/ml of LTA directly in spiked human serum. We intend to combine this rapid assay with others currently in development into a diagnostic tool for point-of-care detection of bacterial pathogens.</p>
収録刊行物
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- Transactions of the Materials Research Society of Japan
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Transactions of the Materials Research Society of Japan 42 (4), 101-105, 2017
一般社団法人 日本MRS
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詳細情報
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- CRID
- 1390001205511627648
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- NII論文ID
- 130005878468
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- ISSN
- 21881650
- 13823469
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可