Development of Time-course Oxygen Binding Analysis for Hemoglobin-based Oxygen Carriers

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Author(s)

Abstract

Developing blood substitutes is in urgent demand for chronic blood shortage all over the world. In this connection, the oxygen binding behavior of hemoglobin-based oxygen carriers (HBOCs) is one of the most important characteristics. However, present methods available for estimating oxygen binding behavior have need of expensive apparatus, and also are not suitable for high-throughput and the time-course analysis. To overcome these problems, we proposed a simple analysis method for the time-course oxygen binding behavior of HBOCs, which employs a general UV-Vis microplate reader and a common reagent, sodium dithionite, as a reductant for HBOCs and an oxygen scavenger. Our method enabled time-course oxygen binding behavior analysis of HBOCs in a simple manner, and obtained data corresponding with those by the conventional method. Thus, our developed method will accelerate the development of HBOCs due to easy oxygen binding analysis.

Journal

  • Analytical Sciences

    Analytical Sciences 33(8), 953-956, 2017

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130005950153
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    028444236
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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