<b>Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical </b><b>bone </b>

• SAKURAI Atsunaka

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Restorative Dentistry, Graduate School of Dental Medicine, Hokkaido University

• HASEGAWA Tomoka

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• KUDO Ai

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Crown and Bridge Prosthodontics, Graduate School of Dental Medicine, Hokkaido University

• SHEN Zhao

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University

• NAGAI Tomoya

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Oral Functional Prosthodontics, Graduate School of Dental Medicine, Hokkaido University

• ABE Miki

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• YOSHIDA Taiji

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• HONGO Hiromi

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• YAMAMOTO Tomomaya

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Self- Defense Force Hanshin Hospital

• YAMAMOTO Tsuneyuki

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• ODA Kimimitsu

  Biochemistry, Niigata University Graduate School of Medical and Dental Sciences

• FREITAS Paulo Henrique Luiz de

  Department of Dentistry, Federal University of Sergipe at Lagarto

• LI Minqi

  Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University

• SANO Hidehiko

  Department of Restorative Dentistry, Graduate School of Dental Medicine, Hokkaido University

• AMIZUKA Norio

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

抄録

To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal trabeculae for assessing sclerostin and FGF23 localization: close to the chondro-osseous junction, i.e., bone modeling site even in the adult animals, and the trabecular region distant from the growth plate, where bone remodeling takes place. As a consequence, sclerostin-immunopositive osteocytes could not be observed in both close and distant trabecular regions early at the embryonic and young adult stages. However, osteocytes gradually started to express sclerostin in the distant region earlier than in the close region of the trabeculae. Immunoreactivity for FGF23 was observed mainly in osteoblasts in the early stages, but detectable in osteocytes in the later stages of growth in trabecular and cortical bones. Fgf23 was weakly expressed in the embryonic and neonatal stages, while the receptors, Fgfr1c and αKlotho were strongly expressed in femora. At the adult stages, Fgf23 expression became more intense while Fgfr1c and aKlotho were weakly expressed. These findings suggest that sclerostin is secreted by osteocytes in mature bone undergoing remodeling while FGF23 is synthesized by osteoblasts and osteocytes depending on the developmental/growth stage. In addition, it appears that FGF23 acts in an autocrine and paracrine fashion in fetal and neonatal bones.

収録刊行物

• Biomedical Research

  Biomedical Research 38 (4), 257-267, 2017

  バイオメディカルリサーチプレス

キーワード

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

詳細情報

• CRID: 1390282679878157952
• NII論文ID: 130005966977
• NII書誌ID: AA00110128
• DOI: 10.2220/biomedres.38.257
• ISSN: 1880313X
• ISSN: 03886107
• HANDLE: 2115/72290
• PubMed: 28794403
• Web Site: https://www.jstage.jst.go.jp/article/biomedres/38/4/38_257/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018114706
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可