Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells <i>in vitro</i>: possible involvement of interferon tau as an intermediator

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著者

    • TALUKDER Anup K. TALUKDER Anup K.
    • Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan|Department of Gynecology, Obstetrics and Reproductive Health, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
    • RASHID Mohammad B. YOUSEF Mohamed S.
    • Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan|Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
    • AWAI Kensuke RASHID Mohammad B.
    • Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan|Department of Physiology and Pharmacology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
    • SHIMIZU Takashi ACOSTA Tomas J.
    • Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
    • OKUDA Kiyoshi SHIMIZU Takashi
    • Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
    • SHIMADA Masayuki OKUDA Kiyoshi
    • Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan|Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan
    • MIYAMOTO Akio IMAKAWA Kazuhiko
    • Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki 319-0206, Japan

抄録

<p> Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) <i>in vitro</i>, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced interferon (IFN)-stimulated genes (<i>ISGs</i>: <i>ISG15</i>, <i>OAS1</i>, and <i>MX2</i>), a key factor for IFN-signaling (<i>STAT1</i>), and type-1 IFN receptors (<i>IFNAR1</i> and <i>IFNAR2</i>), with suppression of <i>NFkB2</i>, <i>NFkBIA</i> and pro-inflammatory cytokines (<i>TNFA</i> and <i>IL1B</i>). The embryo also stimulated <i>PTGES</i> and PGE2 secretion in BUECs. In PBMCs, both CM from embryo-BUEC co-culture and embryo culture alone induced <i>ISGs</i>, <i>STAT1</i> and <i>TGFB1</i>, while suppressing <i>TNFA</i> and <i>IL17</i>. Similarly, interferon tau (IFNT) at 100 pg/ml suppressed <i>NFkB2</i>, <i>TNFA</i> and <i>IL1B</i> in BUECs, and also stimulated <i>TGFB1</i> and suppressed <i>TNFA</i> in PBMCs. Our findings suggest that the bovine embryo, in the first four days in the uterus (D5-D9), starts to induce an anti-inflammatory response in epithelial cells and in immune cells. IFNT is likely to act as one of the intermediators for induction of the anti-inflammatory response in the bovine uterus.</p>

収録刊行物

  • 家畜繁殖研究會誌

    家畜繁殖研究會誌 63(4), 425-434, 2017

    日本繁殖生物学会

各種コード

  • NII論文ID(NAID)
    130005982816
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • ISSN
    0916-8818
  • NDL 記事登録ID
    028458173
  • NDL 請求記号
    Z54-H305
  • データ提供元
    NDL  J-STAGE 
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