Hi-C Revolution: From a Snapshot of DNA–DNA Interaction in a Single Cell to Chromosome-Scale <i>De Novo</i> Genome Assembly

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Author(s)

    • Hoshino Arata
    • Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
    • Sakamoto Takuya
    • Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
    • Matsunaga Sachihiro
    • Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science|Research Institute for Science and Technology, Tokyo University of Science

Abstract

<p>Hi-C (high-resolution chromosome conformation capture) provides the genome-wide interactions of all genomic regions. Hi-C can identify topologically associating domains and enhancer-promoter loops. Recently, Hi-C data could be acquired from only a single cell or a single-nucleus. These new approaches enable us to identify the character of individual cells. Moreover, Hi-C is also applicable for the construction of long-range scaffolding of chromosome-scale <i>de novo</i> genome assembly. The chromosome-scale assembly with Hi-C enables us to reveal the spatial chromatin organization in repetitive sequence-rich genomes and validate chromosome rearrangements including chromosome fusions and translocations. In the future, Hi-C will contribute to the determination of an optimal strategy in cancer therapy.</p>

Journal

  • cytologia

    cytologia 82(3), 223-226, 2017

    Japan Mendel Society, International Society of Cytology

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