Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

• Tatsuta Yukina

  Department of Bioscience, Fukui Prefectural University

• Tanaka Yukie

  University of Fukui

• Ikeda Akari

  Taiyo Nippon Sanso Co.

• Matsukawa Shigeru

  University of Fukui

• Katano Hajime

  Department of Bioscience, Fukui Prefectural University

• Taira Shu

  Department of Bioscience, Fukui Prefectural University

Abstract

We compared two ionization methods, matrix assisted laser desorption/ionization (MALDI) and nanoparticle assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS), for the analysis of amino acids derivatized with Py-Tag™ that consists pyrylium-based compound. Py-Tag is a useful stable derivatization reagent due to wide mass differences (using ¹³C as the sole stable labelling isotope). For Py-Tag labelled lysine, sensitive signals that showed less noise with a ten times higher sensitivity, showed a wider mass difference by Nano-PALDI MS compared to MALDI MS.

Journal

• Mass Spectrometry

  Mass Spectrometry 6 (3), S0069-S0069, 2017

  The Mass Spectrometry Society of Japan

Keywords

• proteomics
• Py-Tag
• LC-MS
• stable isotope

Details

• CRID: 1390001205417163392
• NII Article ID: 130006078754
• NII Article ID: 120006501580
• DOI: 10.5702/massspectrometry.s0069
• ISSN: 21865116
• ISSN: 2187137X
• Web Site: https://www.jstage.jst.go.jp/article/massspectrometry/6/3/6_S0069/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed