Temperature measurement in microchannel liquid flow by measuring fluorescence polarization

• SUZUKI Atsushi

  Department of Mechanical Engineering and Science, Kyoto University

• TATSUMI Kazuya

  Department of Mechanical Engineering and Science, Kyoto University

• HORII Satoshi

  Department of Mechanical Engineering and Science, Kyoto University

• KURIYAMA Reiko

  Department of Mechanical Engineering and Science, Kyoto University

• NAKABE Kazuyoshi

  Department of Mechanical Engineering and Science, Kyoto University

Bibliographic Information

Other Title

• 蛍光偏光測定によるマイクロ流路内液体温度計測

Abstract

A fluid temperature measurement technique based on fluorescence polarization is developed and applied to measure the two-dimensional temperature distributions in microchannel. In this measurement method, the fluorescence depolarization due to rotational Brownian motion of the fluorescent molecules in the solution is measured and converted to fluid temperature. Since the fluorescence polarization degree is independent to fluorescence intensity, the measurement is less influenced by the fluorescence quenching effect, which is an issue in laser-induced fluorescence (LIF) method. Experiments were performed using a microchannel with fluorescent molecules solved in water. The effects of the fluorescent molecule concentration, fluid pH and fluid temperature on the fluorescence polarization degree are discussed to evaluate the influence of the quenching effects and to derive the correlation curves. Furthermore, the proposed method was applied to measure the temperature distribution with linear gradient generated in the microchannel. The results showed that the fluorescence polarization is considerably less sensitive to quenching factors compared with the fluorescence intensity measurements. A linear correlation between the polarization degree and the fluid temperature was obtained. This relationship agreed well with the theoretical one. Further, measurement of two-dimensional temperature distribution in the microchannel agreed well with the values obtained by the thermocouple measurements. These results confirmed the validity of the measurements and feasibility of the proposed method.

Journal

• Transactions of the JSME (in Japanese)

  Transactions of the JSME (in Japanese) 83 (853), 17-00200-17-00200, 2017

  The Japan Society of Mechanical Engineers

Keywords

• Fluorecence polarization
• Microscopic measurement
• Temperature measurement
• Liquid
• Brownian motion

Details

• CRID: 1390001205516929792
• NII Article ID: 130006099959
• DOI: 10.1299/transjsme.17-00200
• ISSN: 21879761
• Web Site: https://www.jstage.jst.go.jp/article/transjsme/83/853/83_17-00200/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed