A new model of skeletal muscle atrophy induced by immobilization using a hook-and-loop fastener in mice

• Aihara Masahiro

  Department of Physical Therapy, Faculty of Medical Sciences, Teikyo University of Science: 2525 Yatsuzawa, Uenohara, Yamanashi 409-0193, Japan Division of Physical Therapy, Department of Health Science, International University of Health and Welfare Graduate School, Japan

• Hirose Noboru

  Department of Physical Therapy, Faculty of Medical Sciences, Teikyo University of Science: 2525 Yatsuzawa, Uenohara, Yamanashi 409-0193, Japan Department of Neurology, Teikyo University School of Medicine, Japan

• Katsuta Wakana

  Division of Biosciences, Graduate School of Science and Engineering, Teikyo University of Science, Japan Department of Rehabilitation, National Center of Neurology and Psychiatry, Japan

• Saito Fumiaki

  Department of Neurology, Teikyo University School of Medicine, Japan

• Maruyama Hitoshi

  Division of Physical Therapy, Department of Health Science, International University of Health and Welfare Graduate School, Japan

• Hagiwara Hiroki

  Department of Physical Therapy, Faculty of Medical Sciences, Teikyo University of Science: 2525 Yatsuzawa, Uenohara, Yamanashi 409-0193, Japan Department of Neurology, Teikyo University School of Medicine, Japan Division of Biosciences, Graduate School of Science and Engineering, Teikyo University of Science, Japan

抄録

[Purpose] To study muscle atrophy, the muscle atrophy model mice have been used frequently. In particular, cast immobilization is the most common method to induce muscle atrophy. However, it is time consuming and often causes adverse events including skin injury, edema, and necrosis. The present study, we developed a hook-and-loop fastener (Velcro) immobilization method as a new, simple, and less invasive approach to induce muscle atrophy. [Subjects and Methods] Mice were bandaged in the knee joint extension and ankle plantar extension position. Muscle atrophy was induced by either winding a cast or Velcro around the limb. [Results] According to weight and fiber size, Velcro immobilization induced equivalent muscle atrophy to cast immobilization. Velcro immobilization reduced significantly the time for the procedure and the frequency of adverse events. [Conclusion] Velcro immobilization can induce muscle atrophy comparable to cast immobilization, but in a shorter time and with less complications. Velcro immobilization may contribute to the study of disuse muscle atrophy in clinical practice of physical therapy using a mouse model.

収録刊行物

• Ｊ　Ｐｈｙｓ　Ｔｈｅｒ　Ｓｃｉ

  Ｊ　Ｐｈｙｓ　Ｔｈｅｒ　Ｓｃｉ 29 (10), 1779-1783, 2017

  理学療法科学学会

キーワード

• Skeletal muscle atrophy
• Cast immobilization
• Velcro immobilization

詳細情報

• CRID: 1390001204330633600
• NII論文ID: 130006178267
• DOI: 10.1589/jpts.29.1779
• ISSN: 21875626
• ISSN: 09155287
• Web Site: https://www.jstage.jst.go.jp/article/jpts/29/10/29_jpts-2017-302/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可