A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy

• Koike Taro

  Department of Anatomy and Cell Science, Kansai Medical University

• Kataoka Yosky

  Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center Cellular Function Imaging Team, Division of Bio-function Dynamics Imaging, RIKEN Center for Life Science Technologies

• Maeda Mitsuyo

  Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center

• Hasebe Yuji

  Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center

• Yamaguchi Yuuki

  Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center

• Suga Mitsuo

  Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center

• Saito Akira

  Institute of Biomedical Science Central Research Center, Kansai Medical University

• Yamada Hisao

  Department of Anatomy and Cell Science, Kansai Medical University

Abstract

“Array tomography” is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat. Using this device, we succeeded in mounting several ribbons consisting a mean of 205.6 (SD: 37.7) serial ultrathin sections on 1.25 (SD: 0.06) × 1.25 (SD: 0.06)-cm silicon substrates. Furthermore, it was confirmed that our method is suitable for ribbons derived from water-soluble resin blocks. We were also able to stain the specimens by post-embedding immunocytochemistry. Thus, our method is useful in mounting numerus sections on a substrate for array tomography with SEM.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 50 (5), 135-140, 2017

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• array tomography
• three-dimensional observation
• serial ultrathin section
• post-embedding labeling
• scanning electron microscopy

Details

• CRID: 1390001204861998080
• NII Article ID: 130006179490
• DOI: 10.1267/ahc.17013
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/50/5/50_17013/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018363266
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed