マイクロ市松パターンが筋芽細胞の配向に与える影響

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  • Effect of micro checkered pattern on orientation of myoblast

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<p>Control technique of orientation of a biological cell is expected to be applied to tissue engineering. It is known that morphology of the scaffold affects the cell behavior. For example, the micro stripe pattern can control cell orientation. The effect of aspect ratio of the micro checkered pattern on cell orientation has been investigated in the present study. The dimension of each unit of the micro checkered pattern is as follows: the length is 10 μm, and the height is 0.7 μm. Variation has been made on the width of the micro pattern: 5 μm (A), 8 μm (B) and 10 μm (C). Each type of repeated micro pattern was formed in the square area of 500 μm × 500 μm. The micro pattern was made of the negative photoresist material (SU-8 2) coated on a glass plate by the photolithography technique. C2C12 (mouse myoblast cell) was cultured for 24 hours on the micro pattern. The angle between the longitudinal axis of each cell and the width of the micro pattern was measured on the microscopic image. In each pattern, the average angle was as follows: 62.8 degrees on A, 50.2 degrees on B and 41.2 degrees on C. The experimental results show that the aspect ratio of micro checkered pattern controls cell orientation.</p>

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