Nitrite Activates 5′AMP-Activated Protein Kinase-Endothelial Nitric Oxide Synthase Pathway in Human Glomerular Endothelial Cells

Access this Article

Author(s)

    • Miyamoto Licht Miyamoto Licht
    • Laboratory of Pharmacology and Physiological Sciences, Frontier Laboratory for Pharmaceutical Sciences, Institute of Biomedical Sciences, University of Tokushima Graduate School|Department of Medical Pharmacology, Institute of Biomedical Sciences, University of Tokushima Graduate School
    • Hatano Aya Kono Mai
    • Department of Medical Pharmacology, Institute of Biomedical Sciences, University of Tokushima Graduate School
    • Xu Wenting Kawasaki Aya
    • Department of Medical Pharmacology, Institute of Biomedical Sciences, University of Tokushima Graduate School
    • Ikeda Yasumasa Hatano Aya
    • Department of Medical Pharmacology, Institute of Biomedical Sciences, University of Tokushima Graduate School

Abstract

<p>Recent studies have shown that orally supplied nitrates, which substantially exist in our daily diets, are reduced into nitrites and become significant sources of nitric oxide (NO) especially in hypoxic tissues. However, physiological significance of nitrites in normal tissues has not been elucidated though our serum concentrations of nitrites reach as high as micromolar levels. We investigated effects of nitrite on endothelial NO synthase (eNOS) using human glomerular endothelial cells to reveal potential glomerular-protective actions of nitrites with its underlying molecular mechanism. Here we demonstrate that nitrite stimulation evokes eNOS activation which is dependent on 5′AMP-activated protein kinase (AMPK) activation in accordance with ATP reduction. Thus, nitrites should facilitate AMPK–eNOS pathway in an energy level-dependent manner in endothelial cells. The activation of AMPK–eNOS signals is suggested to be involved in vascular and renal protective effects of nitrites and nitrates. Nitrites may harbor beneficial effects on metabolic regulations as AMPK activators.</p>

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 40(11), 1866-1872, 2017

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130006191905
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    028601993
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
Page Top