Analysis of Fresh Sap Collected from Ryukyu Lacquer Tree

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  • LU Rong
    Department of Applied Chemistry, School of Science and Technology, Meiji University
  • HONDA Takayuki
    Department of Applied Chemistry, School of Science and Technology, Meiji University
  • SUNG Meesook
    Department of Biochemistry, Institute for Clinical and Translational Research, College of Medicine, Catholic Kwandong University
  • JUNG Jaekook
    Department of Architecture, College of Engineering, Catholic Kwandong University
  • MIYAKOSHI Tetsuo
    Department of Applied Chemistry, School of Science and Technology, Meiji University

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Abstract

The chemical structure of fresh lacquer sap collected from a lacquer tree growing in Nago City of Okinawa, Japan, was analyzed by gas chromatography/mass-spectrometry (GC/MS) and nuclear magnetic resonance (NMR). The results showed that Nago lacquer is laccol lacquer and its major components are 3-(heptadeca-10Z,13E,15E-trienyl)catechol, 3-(heptadeca-10Z,13E-dienyl)catechol, 3-(heptadeca-14Z-enyl)catechol, and 3-(heptadeca-12Z-enyl)catechol, which are similar to the components of Vietnamese lacquer. It showed higher laccase activity at pH 5 – 8 and better low temperature adaptability than Vietnamese lacquer. The Nago lacquer reached a dust free dry (DF) condition after 6 h, but Vietnamese lacquer did not. However, both were able to achieve harden dry (HD) in 24 h at 25°C, 80% relative humidity. In order to identify the lacquer provenance, the strontium isotope ratio was analyzed. The strontium isotope ratio (87Sr/86Sr) of Nago lacquer was 0.7110, which is different from the 0.7450 of Vietnamese lacquer.

Journal

  • Analytical Sciences

    Analytical Sciences 33 (11), 1253-1257, 2017

    The Japan Society for Analytical Chemistry

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