Comparative study of cell amount, cell findings and accuracy for conventional smear method and LBC (SurePath method) in oral squamous cell carcinoma cases

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  • KUYAMA Kayo
    Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Pathological Diagnosis, Nihon University Hospital
  • FUTAYA Etsuko
    Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Pathological Diagnosis, Nihon University Hospital
  • UKIGAYA Masayuki
    Diagnostic Pathology, Chiba-Nishi General Hospital
  • MATSUMOTO Takashi
    Pathological Diagnosis, Nihon University Hospital
  • MORIKAWA Miyuki
    Department of Oral Pathology, Nihon University School of Dentistry at Matsudo
  • SUEMITSU Masaaki
    Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Pathological Diagnosis, Nihon University Hospital
  • SAITO Takaaki
    Diagnostic Pathology, Chiba-Nishi General Hospital
  • UTSUNOMIYA Tadahiko
    Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Pathological Diagnosis, Nihon University Hospital
  • SAKAMAKI Hiroyuki
    Department of Dental Hygiene, Chiba Prefectural University of Health Sciences
  • OOMURA Mitsuhiro
    Diagnostic Pathology, Chiba-Nishi General Hospital

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Other Title
  • 口腔扁平上皮癌擦過細胞診における細胞量, 細胞所見および正診性に関する従来法と液状化検体細胞診 (SurePath 法) の比較検討

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Abstract

<p>Objective : The purpose of this study was to compare the cell amount, cell findings and predictive accuracy for squamous cell carcinoma (SCC) between the conventional smear method (CSM) and liquid-based cytology (LBC, SurePath method) using specimens obtained from cases of SCC confirmed by histopathology.</p><p>Study Design : A total of 23 oral SCC smears were processed by the split-sample method (CSM and SurePath). The cell amount, digitization values (cytoplasmic brightness, cell circularity and irregular distribution of chromatin analyzed by ImageJ 1.48), cell findings (background, cluster, cytoplasm and nuclear) and predictive accuracy for SCC were compared.</p><p>Results : The cell amount was significantly higher in the specimen processed by the SurePath method than by CSM. Higher cytoplasmic brightness and irregular distribution of chromatin were observed in the specimens processed by the CSM and SurePath methods, respectively. The cell circularity was equivalent between the two methods. Overlap of cells in the background was avoided to sink in cell classification, and screening became easy for SurePath method. However, the jagged shape of the cell periphery peculiar to the superficial atypical cells seen in the specimens processed by CSM was not observed in those processed by the SurePath method, whereas the nuclear transparency in the basal atypical cells was more pronounced. The predictive accuracies for SCC of specimens processed by CSM and SurePath were not significantly different (62.0% and 61.9%, respectively).</p><p>Conclusion : SurePath method has the advantage of yielding greater cell amounts than CSM. The predictive accuracy for SCC was not significantly different, although peculiar cytological findings were observed in both specimens processed by CSM and LBC.</p>

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