Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage

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Author(s)

    • NISHIMURA Ryo
    • Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan|Laboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • HISHINUMA Mitsugu
    • Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • OKUDA Kiyoshi
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan|Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
    • HASEGAWA Hiroki
    • Laboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • YAMASHITA Masamichi
    • Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • ITO Norihiko
    • Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • OKAMOTO Yoshiharu
    • Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • TAKEUCHI Takashi
    • Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan
    • KUBO Tomoaki
    • United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan
    • IGA Kosuke
    • Division of Livestock and Forage Research, Beef Cattle Production Group, Tohoku Agricultural Research Center, NARO, Iwate 020-0198, Japan
    • KIMURA Koji
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan

Abstract

<p>A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined <i>GLUT1</i> mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0–25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. <i>GLUT1</i> mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O<sub>2</sub>) increased <i>GLUT1</i> mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0–25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.</p>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 79(11), 1878-1883, 2017

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130006206584
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • Data Source
    J-STAGE 
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