Effects of the deletion of <i>hup</i> genes encoding the uptake hydrogenase on the activity of hydrogen production in the purple photosynthetic bacterium <i>Rubrivivax gelatinosus</i> IL144
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- Sato Takeshi
- Department of Biological Sciences, Kanagawa University
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- Inoue Kazuhito
- Department of Biological Sciences, Kanagawa University Research Institute for Photobiological Hydrogen Production, Kanagawa University
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- Sakurai Hidehiro
- Research Institute for Photobiological Hydrogen Production, Kanagawa University
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- Nagashima Kenji V. P.
- Research Institute for Photobiological Hydrogen Production, Kanagawa University
抄録
<p>The efficiency of hydrogen gas production by nitrogenase in bacteria has been improved by the inhibition of antagonistic activity by the uptake hydrogenase. In this study, a mutant lacking the gene coding for the uptake hydrogenase was generated from the photosynthetic beta-proteobacterium Rubrivivax gelatinosus IL144 to explore new ways of hydrogen gas production driven by light energy. The mutant cells produced 25–30% higher amounts of molecular hydrogen than the wild-type cells under nitrogen-deficient conditions under light. Furthermore, by the addition of 5 mM glutamate, the photosynthetic growth rate was greatly enhanced, and the hydrogen gas production activity reached 41.1 (mmol/l) in the mutant.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 63 (5), 274-279, 2017
公益財団法人 応用微生物学・分子細胞生物学研究奨励会