Ethanol extracts of <i>Aster yomena</i> (Kitam.) Honda inhibit adipogenesis through the activation of the AMPK signaling pathway in 3T3-L1 preadipocytes
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- Han Min Ho
- National Marine Biodiversity Institute of Korea
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- Jeong Ji-Suk
- Gurye Wild Flower Institute and Gurye-gun Agricultural Center
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- Jeong Jin-Woo
- Anti-Aging Research Center, Dongeui University Open Laboratory for Muscular and Skeletal Disease, and Department of Biochemistry, Dongeui University College of Korean Medicine
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- Choi Sung Hyun
- Department of System Management, Korea Lift College
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- Kim Sung Ok
- Department of Food Science & Biotechnology, College of Engineering, Kyungsung University
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- Hong Su Hyun
- Open Laboratory for Muscular and Skeletal Disease, and Department of Biochemistry, Dongeui University College of Korean Medicine
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- Park Cheol
- Department of Molecular Biology, College of Natural Sciences, Dongeui University
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- Kim Byung Woo
- Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dongeui University
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- Choi Yung Hyun
- Anti-Aging Research Center, Dongeui University Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dongeui University
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Abstract
<p>The leaves of Aster yomena (Kitam.) Honda have long been used as a traditional herb for treating disorders including coughs, asthma, and insect bites. According to recent studies, A. yomena leaf extracts have several pharmacological properties, including anti-inflammatory, antioxidant, and anti-asthmatic activities. However, little information is available regarding their anti-obesity effect. In this study, we investigated the inhibitory effect of the ethanol extracts of A. yomena leaves (EEAY) on adipocyte differentiation and adipogenesis using 3T3-L1 preadipocytes. When 3T3-L1 preadipocytes were treated with various concentrations of EEAY (ranging from non-toxic), the number of lipid droplets, lipid content, and triglyceride production, the typical characteristics of adipocytes, were suppressed in a concentration-dependent manner. During this process, EEAY significantly reduced the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein α and β, and sterol regulatory element-binding protein-1c. In addition, EEAY was also found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein and leptin. In particular, EEAY treatment effectively enhanced the activation of the AMP-activated protein kinase (AMPK) signaling pathway; however, the co-treatment with compound C, an inhibitor of AMPK, significantly restored the EEAY-induced inhibition of pro-adipogenic transcription factors and adipocyte-specific genes. These results indicate that EEAY may exert an anti-obesity effect by controlling the AMPK signaling pathway, suggesting that the leaf extract of A. yomena may be a potential anti-obesity agent.</p>
Journal
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 11 (5), 281-287, 2017
International Research and Cooperation Association for Bio & Socio-Sciences Advancement
