Characterization of the chemical structure and innate immune-stimulating activity of an extracellular polysaccharide from <i>Rhizobium</i> sp. strain M2 screened using a silkworm muscle contraction assay
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- Urai Makoto
- Graduate School of Pharmaceutical Sciences, The University of Tokyo Department of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture
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- Aizawa Tomoko
- Department of Bioscience in Daily Life, College of Bioresource Sciences, Nihon University
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- Imamura Katsutoshi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo Department of Microbiology and Molecular Genetics, Graduate School of Pharmaceutical Sciences, Chiba University
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- Hamamoto Hiroshi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo Teikyo University Institute of Medical Mycology
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- Sekimizu Kazuhisa
- Graduate School of Pharmaceutical Sciences, The University of Tokyo Genome Pharmaceuticals Institute Co., Ltd. Teikyo University Institute of Medical Mycology
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抄録
<p>We screened innate immunostimulant-producing bacteria using a silkworm muscle contraction assay, and isolated Rhizobium sp. strain M2 from soil. We purified the innate immunostimulant from strain M2, and characterized the chemical structure by nuclear magnetic resonance spectroscopy and chemical analyses. The innate immunostimulant (M2 EPS) comprised glucose, galactose, pyruvic acid, and succinic acid with a molar ratio of 6.8:1.0:0.9:0.4, and had a succinoglycan-like high molecular-weight heteropolysaccharide structure. To determine the structural motif involved in the innate immunostimulating activity, we modified the M2 EPS structure chemically, and found that the activity was increased by removal of the succinic and pyruvic acid substitutions. Strong acid hydrolysis completely inactivated the M2 EPS. Unmasking of the β-1,3/6-glucan structure of the side-chain by deacylation and depyruvylation may enhance the innate immune-stimulating activity of M2 EPS. These findings suggest that the succinoglycan-like polysaccharide purified from strain M2 has innate immune-stimulating activity, and its glycan structure is necessary for the activity.</p>
収録刊行物
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 11 (5), 238-245, 2017
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会