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- Tani Hidenori
- Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Takeshita Jun-ichi
- Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST)
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- Aoki Hiroshi
- Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Nakamura Kaoru
- Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Abe Ryosuke
- College of Engineering Systems, School of Science and Engineering, University of Tsukuba
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- Toyoda Akinobu
- College of Engineering Systems, School of Science and Engineering, University of Tsukuba
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- Endo Yasunori
- Department of Risk Engineering, Faculty of Systems and Information Engineering, University of Tsukuba
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- Miyamoto Sadaaki
- Department of Risk Engineering, Faculty of Systems and Information Engineering, University of Tsukuba
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- Gamo Masashi
- Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST)
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- Torimura Masaki
- Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Sato Hiroaki
- Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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抄録
<p>Several chemicals, such as methyl p-hydroxybenzoate (MHB), have been widely used as preservatives in the water baths of CO2 incubators used for mammalian cell culture, and they are not considered to produce any biological effects. However, no detailed analyses of the effects of these compounds on cultured cells have been reported. In this study, we thus examined whether MHB in the incubator water bath affects cell viability or genome-wide gene expression in mouse embryonic stem cells under control conditions [using only dimethyl sulfoxide (DMSO) in the culture medium] and under chemical-treated conditions using benzene and chloroform; conditions that simulate a cell-based toxicity assay. We found that (i) MHB significantly altered cell growth rate, and (ii) MHB affected gene expression levels related to pathways that modulate cell growth and basic molecular processes, not only under control conditions but also the chemical-treated conditions. Furthermore, Gene Ontology term analyses revealed that the effects of MHB cannot be accounted for by subtracting the gene expression pattern in the control conditions from that in the chemical-treated conditions. Thus, we suggest that the use of MHB or other preservatives in a CO2 incubator water bath is reconsidered in terms of potential confounding effects on cultured cells.</p>
収録刊行物
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 11 (5), 276-280, 2017
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会