Sequential Formation of Asymmetric Lipid Vesicles for Investigation of Biomolecule Interactions

DOI Web Site

Bibliographic Information

Other Title
  • 人工細胞膜内の生体分子運動を観察可能とする非対称膜リポソームの連続的作製法の開発

Abstract

<p>This paper describes sequential formation of asymmetric lipid vesicle, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by split and contact of droplets. Asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with our single device using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top