In vivo Incorporation of an Alkyne-Bearing Amino Acid into Bombyx mori Silk Fibroin
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Bombyx mori silk fibroin is a natural protein material characterized by mechanical toughness and biocompatibility, which makes it a promising candidate for the development of biomaterials. To adapt silk fibroin to a diverse range of applications, versatile methods for tuning its properties are desirable. Here, we report the in vivo incorporation of an alkyne-bearing unnatural amino acid, 4-ethynylphenylalanine (EthPhe), into B. mori silk fibroin and demonstrate that EthPhe can be used as a selective chemical handle for conjugation with functional molecules by click chemistry. EthPhe-incorporated silk fibroin (AlkyneSilk) is produced by oral administration of EthPhe to transgenic B. mori larvae expressing a mutant of the B. mori phenylalanyl-tRNA synthetase α-subunit in posterior silk glands. We verified that ethynyl groups in AlkyneSilk selectively react with azide-bearing molecules, demonstrating the utility of AlkyneSilk as a “clickable” silk material which can be easily decorated with desired functional molecules.<br>
収録刊行物
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 86 (3), 3_113-3_121, 2017
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173775104
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- NII論文ID
- 130006223755
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 028638499
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可