Galactose oxidase/kelch repeat-containing protein is involved in the iron deficiency stress response in the roots of Hyoscyamus albus Galactose oxidase/kelch repeat-containing protein is involved in the iron deficiency stress response in the roots of <i>Hyoscyamus albus</i>

Access this Article

Author(s)

    • Kawahara Yuki
    • Graduate School of Fisheries and Environmental Sciences, Nagasaki University
    • Nakayama Hideki
    • Graduate School of Fisheries and Environmental Sciences, Nagasaki University
    • Kitamura Yoshie
    • Graduate School of Fisheries and Environmental Sciences, Nagasaki University

Abstract

To confirm the involvement of galactose oxidase/kelch repeat-containing protein (Glx) in response to iron (Fe) deficiency in <i>Hyoscyamus albus</i>, we cloned a putative full-length <i>HaGlx</i> cDNA, which contained an open reading frame (975 bp, 324 amino acids). <i>HaGlx</i> was confirmed by homology searches, molecular phylogeny analysis, and domain search. <i>HaGlx</i> was expressed in the roots but not in the leaves, and the expression significantly increased under Fe deficiency. Sequencing of ∼1.9 kb of the 5'-upstream region of the <i>HaGlx</i> gene, followed by the analysis of promoter elements, resulted in the identification of multiple root-specific elements together with stress-induced elements, including the Fe deficiency-induced element (<i>IDE1</i>) core motif. This suggests that <i>HaGlx</i> plays a key role in stress responses induced under Fe deficiency in the roots. To our knowledge, this is the first report confirming, in a plant other than <i>Arabidopsis thaliana</i>, that Glx is involved in the stress response to Fe deficiency.

Journal

  • Plant Root

    Plant Root 11(0), 58-63, 2017

    Japanese Society for Root Research

Codes

  • NII Article ID (NAID)
    130006233706
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1881-6754
  • Data Source
    IR  J-STAGE 
Page Top