Galactose oxidase/kelch repeat-containing protein is involved in the iron deficiency stress response in the roots of <i>Hyoscyamus albus</i>
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To confirm the involvement of galactose oxidase/kelch repeat-containing protein (Glx) in response to iron (Fe) deficiency in <i>Hyoscyamus albus</i>, we cloned a putative full-length <i>HaGlx</i> cDNA, which contained an open reading frame (975 bp, 324 amino acids). <i>HaGlx</i> was confirmed by homology searches, molecular phylogeny analysis, and domain search. <i>HaGlx</i> was expressed in the roots but not in the leaves, and the expression significantly increased under Fe deficiency. Sequencing of ∼1.9 kb of the 5'-upstream region of the <i>HaGlx</i> gene, followed by the analysis of promoter elements, resulted in the identification of multiple root-specific elements together with stress-induced elements, including the Fe deficiency-induced element (<i>IDE1</i>) core motif. This suggests that <i>HaGlx</i> plays a key role in stress responses induced under Fe deficiency in the roots. To our knowledge, this is the first report confirming, in a plant other than <i>Arabidopsis thaliana</i>, that Glx is involved in the stress response to Fe deficiency.
- Plant Root
Plant Root 11(0), 58-63, 2017
Japanese Society for Root Research