A Fluorescence Quenching Assay Based on Molecular Beacon Formation through a Ligase Detection Reaction for Facile and Rapid Detection of Point Mutations

• SAWAMURA Kensuke

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Masahiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

抄録

A fluorescence quenching assay based on a ligase detection reaction was developed for facile and rapid detection of point mutations present in a mixed population of non-variant DNA. If the test DNA carried a targeted mutation, then the two allele-specific primers were ligated to form a molecular beacon resulting in the expected fluorescence quenching signatures. Using this method, we successfully detected as low as 5% mutant DNA in a mixture of wild-type DNA (t test at 99% confidence level).

収録刊行物

• Analytical Sciences

  Analytical Sciences 33 (12), 1457-1460, 2017

  社団法人 日本分析化学会

キーワード

• Fluorescence quenching
• Förster resonance energy transfer
• ligase detection reaction
• molecular beacon
• point mutation
• polymerase chain reaction

詳細情報

• CRID: 1390001204260097152
• NII論文ID: 130006248743
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.33.1457
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028734376
• PubMed: 29225240
• Web Site: http://id.ndl.go.jp/bib/028734376
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028734376
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.1457.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.1457/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/12/33_1457/_pdf
• 本文言語コード: en
• データソース種別:
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  • PubMed
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