Effect of metallothionein on ethanol-induced hepatotoxicity in mice

DOI Web Site 被引用文献1件 参考文献23件
  • Tokumoto Maki
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
  • Shibuya Kiyoshi
    Department of Pharmacy, Kitasato University Medical Center
  • Lee Jin-Yong
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
  • Tohyama Chiharu
    Faculty of Medicine, University of Tsukuba National Institute for Environmental Studies
  • Satoh Masahiko
    Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University National Institute for Environmental Studies

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<p>Metallothionein (MT) is a small, metal-binding protein that can act as a scavenger of free radicals. To determine whether MT is involved in ethanol-induced hepatotoxicity, which is known to occur through oxidative stress, we studied sensitivity to hepatotoxicity caused by ethanol in MT-null mice genetically deleted for MT-I and MT-II. MT-null mice and wild-type mice were i.p. administrated with ethanol (99.5%, 2.0 g/kg). The increase in GPT, GOT, and LDH activities in the serum of MT-null mice was significantly higher than in the wild-type mice 24 hr after ethanol treatment. Histopathological examination in the liver of ethanol-treated MT-null mice demonstrated vacuolar degeneration. In contrast, histopathologic change was less prominent in the liver of ethanol-treated wild-type mice. Moreover, ethanol increased lipid peroxidation levels only in the liver of MT-null mice. These results indicate that deletion of MT is associated with ethanol-induced severe hepatotoxicity through oxidative stress.</p>

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