Bone Marrow Endothelial Cells Induce Immature and Mature B Cell Egress in Response to Erythropoietin

• Ito Takeshi

  Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University

• Hamazaki Yoko

  Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University Center for iPS Cell Research and Application (CiRA), Laboratory of Immunobiology, Graduate School of Medicine, Kyoto University

• Takaori-Kondo Akifumi

  Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University

• Minato Nagahiro

  Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University

抄録

Bone marrow stromal cells, including endothelial cells and mesenchymal stromal cells, support the maintenance, differentiation, and retention of hematopoietic stem and precursor cells under steady state conditions. At the onset of an emergency, such as severe blood loss or infection, the status of hematopoiesis in the bone marrow changes rapidly to ensure efficient production of cells of specific lineages; however, the function of stromal cells in emergency hematopoiesis has not been fully elucidated. Here, we unexpectedly found that B precursor, mature B, and T cells were released from the bone marrow into the blood circulation in the early phase of hemorrhagic anemia and phenylhydrazine-induced hemolytic anemia. Administration of erythropoietin, which normally increases in response to anemia, stimulated the egress of IgD^low immature B cells and recirculating mature B cells, which usually reside in the perivascular and intravascular space, from the bone marrow within 24 h. We also observed that endothelial cells in the bone marrow expressed erythropoietin receptor, and the expression levels were higher than those in other tissues. Erythropoietin stimulation of bone marrow endothelial cells induced the phosphorylation of STAT5 in vitro. Moreover, in vivo treatment with erythropoietin decreased surface VCAM1 expression and Cxcl12 transcription in bone marrow endothelial cells, both of which are essential for immature and mature B cell retention in the bone marrow. These results suggest that bone marrow endothelial cells can sense and rapidly respond to erythropoietin increase during anemia, thereby regulating B cell emigration from the bone marrow during emergency hematopoiesis. Key words: erythropoietin, anemia, endothelial cells, B cell, bone marrow microenvironment

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 42 (2), 149-157, 2017

  日本細胞生物学会

キーワード

• erythropoietin
• anemia
• endothelial cells
• B cell
• bone marrow microenvironment

詳細情報

• CRID: 1390282679672062336
• NII論文ID: 130006251709
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.17018
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 028921380
• PubMed: 29070774
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028921380
• Web Site: https://www.jstage.jst.go.jp/article/csf/42/2/42_17018/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019042877
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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