Stability of Weakly Acidic Hypochlorous Acid Solution with Microbicidal Activity

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Author(s)

    • ISHIHARA MASAYUKI
    • Division of Biomedical Engineering, Research Institute, National Defense Medical College
    • MURAKAMI KAORU
    • Department of Oral and Maxillofacial Surgery, National Defense Medical College
    • FUKUDA KOICHI
    • Division of Biomedical Engineering, Research Institute, National Defense Medical College
    • NAKAMURA SHINGO
    • Division of Biomedical Engineering, Research Institute, National Defense Medical College
    • KUWABARA MASAHIRO
    • Department of Plastic and Reconstructive Surgery, National Defense Medical College
    • HATTORI HIDEMI
    • Department of Biochemistry and Applied Biosciences, University of Miyazaki
    • FUJITA MASANORI
    • Division of Environmental Medicine, Research Institute, National Defense Medical College
    • KIYOSAWA TOMOHARU
    • Department of Plastic and Reconstructive Surgery, National Defense Medical College
    • YOKOE HIDETAKA
    • Department of Oral and Maxillofacial Surgery, National Defense Medical College

Abstract

<p> Hypochlorous acid (HOCl) solution (200 ppm, pH 6) was prepared and evaluated for their stabilities and microbicidal activities. We demonstrated that HOCl is unstable against ultraviolet (UV) light, sunshine, contact with air, and elevated temperature (≧25℃). Furthermore, in the HOCl solution, the presence of excess NH<sub>2</sub>- or CHO-containing organic compounds such as proteins and carbohydrates, or of inorganic ions such as NO<sub>2</sub><sup>-</sup>, SO<sub>3</sub><sup>-</sup>, PO<sub>3</sub><sup>-</sup>, Fe<sup>2+</sup>, Cu<sup>2+</sup>, and CuS, resulted in the rapid consumption of HOCl by oxidation reactions, and significantly decreased the microbicidal activity of the HOCl solution against coliform bacteria and total viable cell count. Thus, production of stable HOCl solution requires formulation in pure water harboring concentrations as low as possible of various compounds and ions, as well as storage in dark and cool conditions (<10℃) to maintain the concentration of HOCl molecules and microbicidal activity.</p>

Journal

  • Biocontrol Science

    Biocontrol Science 22(4), 223-227, 2017

    The Society for Antibacterial and Antifungal Agents, Japan

Codes

  • NII Article ID (NAID)
    130006285032
  • Text Lang
    ENG
  • ISSN
    1342-4815
  • Data Source
    J-STAGE 
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