Characterizations of oral microbiota in elderly nursing home residents with diabetes

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Author(s)

    • Ogawa Taiji
    • Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry
    • Sasaki Satoshi
    • Department of Social and Preventive Epidemiology, School of Public Health, The University of Tokyo
    • Kawabata Shigetada
    • Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry
    • Maeda Yoshinobu
    • Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry
    • Honda-Ogawa Mariko
    • Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry
    • Ikebe Kazunori
    • Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry
    • Kibi Masahito
    • Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry

Abstract

<p>Over 700 bacterial species have been detected in the oral cavity. Several studies have suggested that periodontitis is associated with systemic disorders such as diabetes mellitus, indicating a key role for oral microbiota in human health. However, the relationship between oral microbiota and diabetes has not been well clarified. Therefore, we conducted microbiome analysis of saliva samples obtained from 15 elderly residents (3 with type 2 diabetes mellitus [DM] and 12 without diabetes [non-DM]) at three different nursing homes, as well as 9 young healthy controls (HC). Genomic DNA was extracted from each sample, and then the V4 region of the 16S rRNA gene was amplified and sequenced. Alpha diversity, in terms of operational taxonomic unit richness, was significantly higher in samples from the non-DM group than in those from the HC group. Weighted UniFrac distance analysis showed that salivary microbial communities in the DM group were separately clustered. Furthermore, in the DM group, <i>Actinomyces</i> and <i>Selenomonas</i> showed significantly higher abundance, whereas <i>Alloprevotella</i> showed significantly lower abundance, relative to the non-DM group. Although our findings were limited by the small sample size, oral bacterial diversity in the DM group was clearly different from that in the non-DM group.</p>

Journal

  • Journal of Oral Science

    Journal of Oral Science 59(4), 549-555, 2017

    Nihon University School of Dentistry

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