Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice
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- Iguchi Shinya
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Suzuki Daigo
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Kawano Eisuke
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Mashimo Takayuki
- Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Juntendo University
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- Kajiya Mikihito
- Department of Periodontal Medicine, Applied life Sciences, Institute of Biomedical and Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University
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- Toriumi Taku
- Department of Anatomy, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
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- Kawai Toshihisa
- Department of Periodontology, Nova Southeastern University College of Dental Medicine
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- Kurihara Hidemi
- Department of Periodontal Medicine, Applied life Sciences, Institute of Biomedical and Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University
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- Isokawa Keitaro
- Department of Anatomy, Nihon University School of Dentistry Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry
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- Sato Shuichi
- Department of Periodontology, Nihon University School of Dentistry Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry
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- Honda Masaki
- Department of Oral Anatomy, Aichi Gakuin University School of Dentistry
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Abstract
<p>Bone marrow-derived multipotent stromal cells (BMSCs) have potent antiinflammatory effects. This study aimed to investigate the antiinflammatory potential of BMSCs using a mouse model of ligature-induced periodontitis. BMSCs were isolated from the femurs and tibiae of mice. Periodontitis was induced by placing a ligature around the right maxillary second molar. After 3 days, the mice were administered BMSC in the gingiva of the mesial interdental papilla around the ligatured molar. The ligatured and non-ligatured mice that were not administered BMSC served as controls. Differences in inflammatory infiltration and bone resorption around the roots of the second molar were assessed and were subsequently quantified using microcomputed tomography (micro-CT), histological analysis, and tartrate-resistant acid phosphatase (TRAP) staining. Micro-CT revealed that alveolar bone loss around the ligatured molars increased in a time-dependent manner; however, the effect was significantly less in BMSC-treated mice compared with ligatured control mice. Tissue histopathology revealed that BMSC administration mitigated inflammatory infiltration in ligatured BMSC mice. In addition, the number of TRAP-positive osteoclasts was markedly elevated in ligatured control mice compared with those in BMSC-treated mice. These findings indicate that local BMSC administration can mitigate inflammation and alveolar bone resorption, suggesting that administering BMSC leads to new therapeutics for periodontitis.</p>
Journal
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- Journal of Oral Science
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Journal of Oral Science 59 (4), 629-637, 2017
Nihon University School of Dentistry