Vitrification of Biological Cells Using a Cryogenic Fine Solid Particulate Spray

• ISHIMOTO Jun

  Graduate School of Information Sciences, Tohoku University

抄録

We have developed a cryopreservation method that uses a single-component super-adiabatic two-fluid de Laval nozzle for the continuous production of solid nitrogen particles with a micro-nano-order particle size. The cell viability results showed that use of the solid nitrogen spray improved cell viability at the time of thawing by more than 20% compared with the conventional liquid nitrogen immersion freezing method. By using the present dynamic spray cooling method that avails of the synergistic effect of impingement heat transfer, convective heat transfer, and evaporation latent heat transfer of solid nitrogen particles, the new vitrification method reduces the cell membrane disruption caused by ice nucleation, crystal growth, and volume expansion.

収録刊行物

• Interdisciplinary Information Sciences

  Interdisciplinary Information Sciences 23 (2), 167-170, 2017

  東北大学大学院情報科学研究科ジャーナル編集委員会

キーワード

• cryogenics
• solid nitrogen spray
• vitrification
• cell viability

詳細情報

• CRID: 1390001204437597696
• NII論文ID: 130006287943
• DOI: 10.4036/iis.2017.s.01
• ISSN: 13476157
• ISSN: 13409050
• HANDLE: 10097/00125622
• Web Site: https://tohoku.repo.nii.ac.jp/records/128219
• Web Site: https://www.jstage.jst.go.jp/article/iis/23/2/23_2017.S.01/_pdf
• 本文言語コード: en
• データソース種別:
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